ifn ε antibody  (Novus Biologicals)

Journal: Immunity, Inflammation and Disease

Article Title: Upregulation of IFNE in cervical biopsies of patients with high‐risk human papillomavirus infections

doi: 10.1002/iid3.1111

Figure Lengend Snippet: IFN‐ε is expressed at high levels in cervical tissue regardless of hormonal status. (Α) Cervical biopsy samples ( n = 32) were examined for mRNA levels of IFN‐α2, IFN‐β, IFN‐ε, IFN‐κ, IFN‐ω, IFN‐γ, and IFN‐λ1. (B) IFN expression levels in cervical samples from patients in follicular ( n = 12) and luteal ( n = 13) stage and postmenopausal patients ( n = 3). IFN expression of postpartum patients and patients undergoing GnRH‐α therapy is not shown.

Article Snippet: For immunofluorescence, sections were blocked with 10% goat serum and incubated with IFN‐ε antibody (1 μg/mL, Novus biologicals) or mouse IgG2b (1 μg/mL, BioLengends) for 24 h at 4°C.

Techniques: Expressing

Journal: Immunity, Inflammation and Disease

Article Title: Upregulation of IFNE in cervical biopsies of patients with high‐risk human papillomavirus infections

doi: 10.1002/iid3.1111

Figure Lengend Snippet: IFN‐ε expression is upregulated in HR‐HPV positive patients at the mRNA but not protein level. (A) IFN expression levels in cervical samples from HR‐HPV negative ( n = 10) and HR‐HPV positive ( n = 22) patients (* p < .05). (B) IFN‐ε expression determined in representative samples of HR‐HPV negative ( n = 10) and HR‐HPV positive ( n = 22) patients by immunoblotting. (C) Immunofluorescence staining for IFN‐ε (red) and DAPI (blue) in cervical tissues of HR‐HPV negative and HR‐HPV positive patients. (D) Quantitative analysis of immunofluorescence staining for IFN‐ε in cervical samples of HR‐HPV negative ( n = 4) and HR‐HPV positive ( n = 6) patients. (E) Immunohistochemical analysis of IFN‐ε protein levels in cervical biopsies of HR‐HPV positive and HR‐HPV negative patients.

Article Snippet: For immunofluorescence, sections were blocked with 10% goat serum and incubated with IFN‐ε antibody (1 μg/mL, Novus biologicals) or mouse IgG2b (1 μg/mL, BioLengends) for 24 h at 4°C.

Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Immunohistochemical staining

Journal: Immunity, Inflammation and Disease

Article Title: Upregulation of IFNE in cervical biopsies of patients with high‐risk human papillomavirus infections

doi: 10.1002/iid3.1111

Figure Lengend Snippet: IFN‐ε is expressed at high levels in cervical tissue regardless of hormonal status. (Α) Cervical biopsy samples ( n = 32) were examined for mRNA levels of IFN‐α2, IFN‐β, IFN‐ε, IFN‐κ, IFN‐ω, IFN‐γ, and IFN‐λ1. (B) IFN expression levels in cervical samples from patients in follicular ( n = 12) and luteal ( n = 13) stage and postmenopausal patients ( n = 3). IFN expression of postpartum patients and patients undergoing GnRH‐α therapy is not shown.

Article Snippet: After blocking with 2.5% BSA, membranes were incubated at 4°C for 24 h with IFN‐ε mouse antibody (1:1000, Novus Biologicals) or β‐actin antibody (1:1000, ABclonal).

Techniques: Expressing

Journal: Immunity, Inflammation and Disease

Article Title: Upregulation of IFNE in cervical biopsies of patients with high‐risk human papillomavirus infections

doi: 10.1002/iid3.1111

Figure Lengend Snippet: IFN‐ε expression is upregulated in HR‐HPV positive patients at the mRNA but not protein level. (A) IFN expression levels in cervical samples from HR‐HPV negative ( n = 10) and HR‐HPV positive ( n = 22) patients (* p < .05). (B) IFN‐ε expression determined in representative samples of HR‐HPV negative ( n = 10) and HR‐HPV positive ( n = 22) patients by immunoblotting. (C) Immunofluorescence staining for IFN‐ε (red) and DAPI (blue) in cervical tissues of HR‐HPV negative and HR‐HPV positive patients. (D) Quantitative analysis of immunofluorescence staining for IFN‐ε in cervical samples of HR‐HPV negative ( n = 4) and HR‐HPV positive ( n = 6) patients. (E) Immunohistochemical analysis of IFN‐ε protein levels in cervical biopsies of HR‐HPV positive and HR‐HPV negative patients.

Article Snippet: After blocking with 2.5% BSA, membranes were incubated at 4°C for 24 h with IFN‐ε mouse antibody (1:1000, Novus Biologicals) or β‐actin antibody (1:1000, ABclonal).

Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Immunohistochemical staining